Research:
Ph.D. Project Title:
CHARACTERIZATION AND AUGMENTATION OF THE HEAT SHOCK PROTEIN 70 RESPONSE TO THERMAL STRESS
Our goal was to determine the role and significance of hsp70 protein to the cellular and tissue response after laser injury. Potentially, this work could lead to a better understanding of and more effective treatment protocols for medical applications of the laser. Faster wound healing times, mitigation of collateral damage from the laser and improved aesthetics are all aspects that stand to benefit from this study. In particular, this work focused on delivering a mild thermal pretreatment several hours in advance of a more severe heat stress later on.
In order to examine the behavior of hsp70 in laser/tissue interactions we have used both cell culture and animal models. A knockout cell line that is deficient in hsp70 was compared to a wild type line to demonstrate the necessity of the protein for full thermotolerance from pretreatment. A microarray and real time PCR analysis was also done to survey the collection of genes that are upregulated in the thermal pretreatment. We are now currently investigating an hsp70 knockout mouse to determine if the reliance upon hsp70 still exists in the full animal model by delivering the thermal pretreatment with a diode laser at 1.85 microns for 10 minutes, which produces a temperature of 44 degrees on the dorsal skin of the mouse.
Master’s Thesis:
IMAGING OF OPTICALLY ACTIVE REPORTER GENES TO ASSESS HSP70 GENE EXPRESSION FOLLOWING THERMAL LASER INJURY
We examined the response of the HSP70 gene in murine fibroblasts to Ho:YAG laser thermal stress and then performed Arrhenius analysis of HSP70 transcription to characterize the response in relation to other, more classical metrics of laser damage, such as protein denaturation of tissue.
Heat Shock
Protein (HSP70) Expression in Moderately Stresses Cells
Personal Bio:
Wife: Ashley
Cats: Shackleton & Henry
Interests: Competitive Running, Cycling, Paddling, Rock Climbing
Publications:
Wilmink GJ, Opalenik SR,
Beckham JT,
Abraham A, Nanney LB, Mahadevan-Jansen A, Davidson JM,
Jansen ED, Molecular Imaging-Assisted Optimization of Hsp70 Expression During
Laser Preconditioning for Wound Repair Enhancement. J
Invest Derm (submitted)
Wilmink
GJ, Opalenik SR,
Beckham JT, Mackanos MA, Nanney LB, Contag CH, Davidson
JM, Jansen ED Optical
imaging of hsp70 expression to assess collateral tissue
damage associated with infrared laser ablation of skin.
J Biomed Optics (submitted)
Faley
SL, Takahashi K, Crooke CE,
Beckham JT,
Tomemori T, Shappell SB, Jansen ED, Takahashi T.
Bioluminescence imaging of
vascular endothelial growth factor promoter activity in
murine mammary tumorigenesis.
Mol Imaging. 2007
Sep-Oct;6(5):331-9
Wilmink,
G.J., S. Opalenik, J.T. Beckham, J.M.. Davidson, E. D. Jansen,
Assessing
laser-tissue damage with bioluminescent imaging. J Biomed Opt, 2006. 11(4): p. 041114.
Anderson, C.D., W.C. Lin, J. Beckham,, A.
Mahadevan-Jansen, C.R. Buttemere, J. Pierce,
I.B. Nicoud, C.
Wright Pinson, and R.S. Chari, Fluorescence spectroscopy
accurately detects irreversible cell damage during hepatic
radiofrequency ablation. Surgery, 2004.
136(3): p.
524-31.
Beckham, J.T., M.A. Mackanos, C. Crooke, T. Takahashi, C. O'Connell-Rodwell, C.H.
&
Contag, and E.D. Jansen, Assessment of cellular response
to thermal laser injury through bioluminescence imaging of
heat shock protein 70. Photochem Photobiol, 2004.
79(1): p.76-85.
O’Connell Rodwell, C.E., D. Shriver, D.M. Simanovskii, C.
McClure, Yu-an. Cao, W. Zhang, M.H. Bachmann,
J.T. Beckham, E.D. Jansen, D. Palanker, H.A. Schwettman, and C.H. Contag, A genetic reporter of thermal stress
defines physiologic zones over a defined
temperature range.
FASEB Journal, 2003.
18(2).
Izzo, A.D., M.A. Mackanos,
J.T. Beckham, and
E.D. Jansen, In vivo optical imaging of
expression
of vascular endothelial growth factor following laser
incision in skin. Lasers Surg Med, 2001.
29(4): p. 343-50.
