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BME 286: Biomedical Optics Lab
Objective | Topics | Grading

Laboratory Objective

This one hour credit section of the course is optional for students who want to gain practical experience in working with light, lasers and optics as it pertains to biomedical applications (and for those who are desperately in need of one more credit hour). The lab will meet one afternoon per week. Students are expected to work together in groups of three or four (if necessary) on the labs. For each lab a brief pre-lab plan has to be written as well as a lab report. Each group is expected to work on these together and turn in one document per group.

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Laboratory Topics

Lab 1 – Geometric Optics

(a) expand and collimate a HeNe laser beam

(b) modeling of experiments using ray tracing software

(c) build a simple microscope

Lab 2 – Reflection / refraction

(a) Snell’s law

(b) total internal reflection

(c) measure index of refraction with optical flat

(d) Brewster’s angle – measure index of refraction

Lab 3 – Interference and diffraction

(a) fringes of constant thickness

(b) measure angle of wedge plate using Haidinger’s fringes

(c) Lloyd’s mirror

(d) Airy discs and aperture function

(e) measure separation of pair of apertures using Fraunhofer’s diffraction

Lab 4 – Interferometry

(a) build Michelson interferometer

(b) measure wavelength of monochromatic light source

Lab 5 – Fiber optics

(a) preparation of fibers

(b) optical coupling and optical delivery

(c) calculate numerical aperture of fiber

Lab 6 – Absorption and scattering

(a) measurement of transmission, reflection, attenuation and scatter in tissues and tissue phantoms

(b) calculate absorption and scattering coefficient using light propagation models

(c) dynamics of optical properties

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Grading / Required Work

Grading will be based on performance in the lab, pre-lab write-ups, lab reports and participation within the group in which you work.

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